Diagnostic Virology Protocols

Diagnostic Virology Protocols - Methods in Molecular Medicine, vol.12
Editor - Stephenson, John R. ; Warnes, Alan
Humana Press; 1998;
pp384;
ISBN: 0896034011
Format - PDF


A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.

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Diagnostic Bacteriology Protocols

Diagnostic Bacteriology Protocols :Methods in Molecular Biology, vol.46
Editor - Howard, Jenny; Whitcombe, David M.
Humana Press; 1995;
pp293;
ISBN: 0896032973


Diagnostic Bacteriology Protocols presents a broad range of currently used techniques for detecting, identifying, and differentiating bacterial cell components, including structured proteins, nucleic acids, enzyme activities, lipopolysaccharides, and metabolites. It describes each technique in simple easy-to-follow steps that guarantee reproducible results for novices and senior researchers alike. Troubleshooting tips, alternative ways of performing procedures, and informative explanations about why certain steps are necessary-aids not usually found in standard journal recipes help even highly skilled researchers to obtain the results they want.

Table of Contents

Antibiotic Resistance Profiling (pp. 1-4)Hammonds, Stephen J.

Bacteriocin Typing (pp. 5-14)Pitt, Tyrone L.; Gaston, Michael A.

Bacteriophage Typing (pp. 15-26)Pitt, Tyone L.; Gaston, Michael A.
The Analysis of Bacterial Proteins by SDS Polyacrylamide Gel Electrophoresis (pp. 27-40)Costas, Menelaos

Lipopolysaccharide Chemotyping (pp. 41-48)Chart, Henrik

Analysis of Bacterial Outer Membrane Proteins (pp. 49-62)Chart, Henrik

Multilocus Enzyme Electrophoresis (pp. 63-78)Boerlin, Patrick; Piffaretti, Jean-Claude

Fast Atom Bombardment-Mass Spectrometry (pp. 79-96)Drucker, David B.

Pyrolysis Mass Spectrometry (pp. 97-106)Freeman, Roger; Sisson, Penelope R.; Heatherington, Clive S.

The Direct Immunofluorescent Filter Technique (DIFT) (pp. 107-112)Kroll, Rohan

The Direct Epifluorescent Filter Technique (DEFT) (pp. 113-122)Kroll, Rohan

Use of Commercially Available ELISA Kits for Detection of Foodborne Pathogens (pp. 123-132)Robison, Barbara J.

Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format (pp. 133-140)Groody, E. Patrick

Preparation of Bacterial Genomic DNA (pp. 141-148)Bickley, Jane; Owen, Robert J.

Pulsed Field Gel Electrophoresis (pp. 149-164)Hillier, Alan J.; Davidson, Barrie E.

Application of Total DNA Restriction Pattern Analysis to Identification and Differentiation of Bacterial Strains (pp. 165-180)Giovannetti, Luciana; Ventura, Stefano

Determination of rRNA Gene Restriction Patterns (pp. 181-200)Grimont, Francine; Grimont, Patrick A. D.

Preparation of Nonradioactive DNA Probes (pp. 201-212)Eley, Adrian; Oxley, Kevin M.

Preparation of Bacterial Plasmid DNA (pp. 213-224)Tully, Michael

Plasmid Profile Typing and Plasmid Fingerprinting (pp. 225-236)Threlfall, E. John; Woodford, Neil

Polymerase Chain Reaction for the Detection of Listeria Species and Listeria monocytogenes (pp. 237-246)Siggens, Kenneth W.

Development of Bacterial Species-Specific DNA Probes Based on Ribosomal RNA Genes Using PCR (pp. 247-256)Smith, Terry J.; Maher, Majella; Gannon, Frank; Dawson, Michael T.

Identification of Microorganisms Using Random Primed PCR (pp. 257-268)Mileham, Alan J.
Ligase Chain Reaction (pp. 269-278)Shimer, George H.; Backman, Keith C.

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Cytomegalovirus Protocols

Cytomegalovirus Protocols : Methods in Molecular Medicine, vol.33
Editor - Sinclair, John
Humana Press; 1999;
pp184;
ISBN: 0896037495
Format - PDF


John Sinclair and a panel of expert investigators present a comprehensive collection of cellular and molecular techniques for the analysis of cytomegalovirus (CMV) biology and its pathogenetic mechanisms. The methods-all described in step-by-step detail with ready reproducibility in mind-range from basic virus culture to complex molecular analysis of CMV structure and function. Included are methods for CMV detection using both immunological and biological techniques, methods for analyzing fundamental aspects of the CMV infection cycle, and methods for analyzing T cell response to cytomegalovirus infection in the human host. Comprehensive and state-of-the-art, Cytomegalovirus Protocols provides investigators with a collection of the key methods that are illuminating not only the basic biology of this complex and intriguing human herpesvirus, but also its significant role in human infectious diseases and their emergent therapies.

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Current Diagnosis & Treatment in Infectious Diseases

Current Diagnosis & Treatment in Infectious Diseases 1st ed
by Walter R. Wilson, W. Lawrence, MD Drew, Nancy K., Phd Henry, Merle A., MD Sande, David A., MD Relman, James M., MD Steckelberg, Julie Louise, MD Gerberding

McGraw-Hill; 2001;
pp985;
ISBN: 0838514944
Format - PDB

Internationally renowned infectious disease experts offer guidance in recognizing, diagnosing, treating, and preventing one of the leading causes of death worldwide. Clinically focused and comprehensive, the book covers the essentials of diagnosis and syndromes in children and adults. Examines antimicrobial resistance and balances expert, practice-oriented coverage with just the right amount of microbiology concepts.

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Handbook of Laboratory Safety

CRC Handbook of Laboratory Safety, Fifth Edition
A. Keith Furr
CRC Press; 2000;
pp808;
ISBN: 0849325234

• OSHA laboratory safety standards
• Guidelines for X-ray use in hospitals
• Enforcement of the standard for dealing with blood-borne pathogens, including the Ryan White Act
• DOT regulations on the packaging of hazardous materials
• EPA action on the release of radioactive materials into the environment
• OSHA actions covering hazardous waste operations and emergency response
• CDC guidelines for research with microbiological hazards

Expanded and updated, The CRC Handbook of Laboratory Safety, Fifth Edition provides information on planning and building a facility, developing an organization infrastructure, planning for emergencies and contingencies, choosing the correct equipment, developing operational plans, and meeting regulatory requirements. Still the essential reference tool, the New Edition helps you organize your safety efforts to adhere to the latest regulations and use the newest technology.

Thoroughly revised, the CRC Handbook of Laboratory Safety, Fifth Edition includes new OSHA laboratory safety standards, the 1994 NRC radiation safety standards, guidelines for X-ray use in hospitals, enforcement of standards for dealing with blood-borne pathogens, OSHA actions covering hazardous waste operations and emergency response, and the latest CDC guidelines for research with microbial hazards. Every word on every page has been scrutinized, and literally hundreds of changes have been made to bring the material up to date.


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Complement Methods and Protocols

Complement Methods and Protocols:Methods in Molecular Biology, vol.150
Ed. Morgan, B. Paul
Humana Press; 2000;
pp280;
ISBN: 0896036545

B. Paul Morgan and a team of expert laboratorians present a comprehensive set of readily reproducible methods to study this critical system. These cutting-edge techniques are suitable both for the basic scientist interested in understanding complement's mechanisms of activation and for the clinical scientist wishing to quantify its activation, and range from the purification of its components to generating complement-deficient mice by gene deletion. Additional techniques presented include procedures for the analysis of its function, for the study of its regulators, for detection of its activation in vivo, and for the identification of its autoantibodies. Comprehensive and cutting-edge, Complement Methods and Protocols offers today's basic and clinical investigators powerful tools for the analysis of the role of complement in human pathophysiology and disease, as well as its therapeutic regulation.

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Chemokine Protocols

Chemokine Protocols:Methods in Molecular Biology, vol.138
Ed. Proudfoot, Amanda E.I.; Wells, Timothy N.C.; Power, Christine
Humana Press; 2000;
pp332;
ISBN: 0896037223


Expert investigators describe in detail the most widely used techniques in chemokine biology. Covering both ligands and receptors, these readily reproducible methods cover all aspects of chemokine research, ranging from the cloning and characterization of chemokines and their receptors, through the use of animal models to study chemokine function in vivo. Each method also includes relevant background information, as well as providing a useful bibliography that renders the study of chemokines accessible at all levels of experience. Comprehensive and highly practical, Chemokine Protocols offers experimental and clinical chemokine researchers today's gold-standard collection of proven methods for analyzing this biologically ubiquitous and important class of proteins

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Biosafety in Microbiological and Biomedical Laboratories

Ed. Jonathan Y. Richmond, Robert W. McKinney
U.S. Department of Health and Human Services; 1999; pp275; ISBN: 0788185136

This publication describes the combinations of standard and special microbiological practices, safety equipment, and facilities constituting Biosafety Levels 1-4, which are recommended for work with a variety of infectious agents in various laboratory settings.

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Microbiological Applications Lab Manual [Benson]

Product details
Spiral-bound: 384 pages
Publisher: McGraw-Hill Publishing Co. (1 Aug 2001)
Language English
ISBN: 0072318899
Size - 32.24 MB
Format - PDF

Book Description
The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and four-color format make Benson's Microbiological Applications: A Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course, Benson assumes no prior organic chemistry course has been taken.

Synopsis
This is the classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and four-color format make Benson's "Microbiological Applications: A Laboratory Manual in General Microbiology" the ideal lab manual. Appropriate for either a majors or non-majors lab course, Benson assumes no prior organic chemistry course has been taken.

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Molecular Cloning: A Laboratory Manual (Sambrook)

Molecular Cloning: A Laboratory Manual
by J. Sambrook, D. Russell

Product details
Hardcover: 295 pages
Publisher: Cold Spring Harbor Laboratory Press,U.S. (5 Dec 2000)
Language English
ISBN: 0879695765
Size - 118MB
Format PDF

In keeping with the style of the first and second editions, the authors have yet again provided the world of molecular biology research with the most comprehensive text book and laboratory manual available. The detailed information is presented in a more text-book format than previous editions, and is closely knit to the experiment protocols and methods. As well as explaining from first principles all of the state of the art laboratory methods you will need in a molecular biology, or related laboratory, the appendix is packed with useful data on enzymes, buffers, biomolecules, a compendum of internet resources and the products of the genomic and post-genomic era: DNA array technology and bioinformatics resources. This millennium edition is truly the molecular biologists' bible for the twenty first century.

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