DNA Microarrays and Gene Expression: From Experiments to Data Analysis and Modeling

DNA Microarrays and Gene Expression: From Experiments to Data Analysis and Modeling
by Pierre Baldi (Author), G. Wesley Hatfield (Author), Wesley G. Hatfield (Author)

Product Details
Hardcover: 230 pages
Publisher: Cambridge University Press; 1 edition (September 30, 2002)
Language: English
ISBN-10: 0521800226

Book Description
Massive data acquisition technologies--such as genome sequencing, high-throughput drug screening, and DNA arrays--are in the process of revolutionizing biology and medicine. This concise, user-friendly and interdisciplinary guide to DNA microarray technology is an introduction and a reference for both biologists and computational scientists. The authors describe the underlying technologies and offer an awareness of the "noise" and pitfalls present in the data generated. They also provide an idea of the different data mining techniques and algorithms that are available to interpret data, and the advantages and disadvantages of each in differing situations.

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Pichia Protocols

Pichia Protocols (Methods in Molecular Biology)
by James M. Cregg (Editor)

Product Details
Hardcover: 268 pages
Publisher: Humana Press; 2nd ed. edition (August 8, 2007)
Language: English
ISBN-10: 1588294293

Book Description
Pichia Protocols focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI).
This volume fully updates and expands upon the first edition of Pichia Protocols, and focuses primarily on information that has come to light since its original publication. Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system.

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Baculovirus and Insect Cell Expression Protocols

Baculovirus and Insect Cell Expression Protocols (Methods in Molecular Biology)
by David W. Murhammer (Editor)

Product Details
Hardcover: 484 pages
Publisher: Humana Press; 2nd ed. edition (July 6, 2007)
Language: English
ISBN-10: 1588295370

Book Description
Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

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Innate Immunity (Methods in Molecular Biology)

Innate Immunity (Methods in Molecular Biology)
by Jonathan Ewbank (Editor), Eric Vivier (Editor)

Product Details
* Hardcover: 300 pages
* Publisher: Humana Press; 1 edition (November 19, 2007)
* Language: English
* ISBN-10: 1588297462

Book Description
Immunologists today are interested in all of the diverse cell-types involved in host defense and have a deeper appreciation of the importance of innate immune mechanisms as a first line of protection against pathogens. Innate Immunity therefore contains chapters dealing with the isolation and functional characterization of cells involved in innate immunity in mouse and man, including mast cells and eosinophils, and with several chapters focusing on natural killer cells. Other chapters describe methods in statistics, in vivo imaging, genome engineering, mutagenesis and culture that are particularly adapted to the study of innate immunity in these hosts. These approaches and models are being used to dissect the complex interplay between hosts and pathogens, and contribute to developing strategies to help fight infection. With chapters written by experts on the cutting-edge of this technology, Innate Immunity is an essential reference for immunologists, histologists, geneticists, and molecular biologists.

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