RT-PCR Protocols

RT-PCR Protocols (Methods in Molecular Biology)
by Joe O'Connell (Editor)

Product Details:
Hardcover: 378 pages
Publisher: Humana Press; 1st edition (July 15, 2002)
Language: English
ISBN: 0896038750

Book Info
National Univ. of Ireland, Cork. Provides techniques used in the molecular investigation of disease pathogenesis and how PT-PCR complements other technological advances in the design of current therapeutic strategies. Contains halftone illustrations. Written in expanded-outline format.

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Current Protocols in Molecular Biology (All Volumes)

Current Protocols in Molecular Biology
Edited by: Fred M. Ausubel (Massachusetts General Hospital and Harvard Medical School); Roger Brent (The Molecular Sciences Institute); Robert E. Kingston (Massachusetts General Hospital and Harvard Medical School); David D. Moore (Baylor College of Medicine); J.G. Seidman (Harvard Medical School); John A. Smith (University of Alabama at Birmingham); Kevin Struhl (Harvard Medical School)
Developmental Editor: Elizabeth W. Harkins

An essential tool for anyone at the forefront of today's molecular biology revolution, Current Protocols in Molecular Biology--the first Current Protocols title--remains the benchmark by which all other lab manuals are judged.

Updated every three months in all formats, Current Protocols in Molecular Biology (CPMB) is constantly evolving to keep pace with the very latest discoveries and developments. The entire collection of published methods plus a year of these quarterly updates are included in the initial purchase price. That's 4900 published pages of content amassed since 1987, plus 670 pages of new (39%) and revised (59%) content over the course of the year! Presently five volumes in its loose-leaf print version, Current Protocols in Molecular Biology...

includes more than 1,000 procedures from hundreds of biologists at top laboratories around the globe.
offers a range of basic methods for DNA preparation to advanced procedures for microarray analysis, chromatin assembly, and more.
covers emerging areas, including mouse phenotyping, DNA construction by recombination, and high-throughput sequencing.

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Molecular Biology Problem Solver: A Laboratory Guide

Molecular Biology Problem Solver: A Laboratory Guide
by Alan S. Gerstein

Paperback: 528 pages
Publisher: Wiley-Liss; 1 edition (August 24, 2001)
Language: English
ISBN: 0471379727

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems.

Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:
Have you selected the most appropriate research strategy?
Have you identified the issues critical to your successful application of a technique?
Are you familiar with the limitations of a given technique?
When should common procedural rules of thumb not be applied?
What strategies could you apply to resolve a problem?

A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:
Working safely with biological samples and radioactive materials
DNA and RNA purification
PCR
Protein and nucleid acid hybridization
Prokaryotic and eukaryotic expression systems
Properly using and maintaining laboratory equipment

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Anti-Tuberculosis Drug Resistance in the World

Anti-Tuberculosis Drug Resistance in the World: Third Global Report
Paperback: 299 pages
Publisher: World Health Organization (March 2005)
Language: English
ISBN: 9241562854

Product Description
The WHO/IUATLD 3rd global report on anti-tuberculosis drug resistance in the world provides data from drug resistance surveys conducted in 77 different countries/geographical settings, between 1999 and 2002. The aim of the project is to collect standardized, reliable and comparable worldwide information on drug resistance of Mycobacterium tuberculosis.

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A Practical Guide to Cellular and Molecular Immunology

TABLE OF CONTENTS

1.0 INFLAMMATION
1.1 Purification of mononuclear and polymorphonuclear cells
1.2 Isolation of cells from the peritoneal cavities of mice
1.3 Adherence purification of monocytes
1.4 Antibody- or C3b-dependent phagocytosis
1.5 Activation of macrophages
1.6 Monokine Bioassays
1.6.1 Assay for IL-1 activity
1.6.2 Assay for IL-6 activity
1.6.3 Cytotoxicity assay for TNFá
1.7. Neutrophil chemotaxis assay
1.8. FcåRI-dependent activation of mast cells
2.0 ANTIBODIES: PURIFICATION & CHARACTERIZATION
2.1 Hybridoma cell culture with production of monoclonal antibodies
2.2 Affinity purification of IgG antibodies
2.2.1 Avid-AL affinity chromatography
2.2.2 Protein A-Sepharose affinity chromatography
2.3 Preparation of IgM antibodies
2.4 Analysis & characterization of immunoglobulins
2.4.1 Polyacrylamide gel electrophoresis of immunoglobulins
2.4.2 Western blotting to detect immunoglobulins
3.0 T CELL AND B CELL RESPONSES
3.1 C'-dependent depletion of CD4+ and CD8+ T Cells
3.2 MACS purification (or depletion) of CD4+ and CD8+ T Cells
3.3 Assessment of T cell proliferation
3.4 Plaque Forming Cell (PFC) assay for IgM-producing cells
3.5 ELISPOT assays for single cytokine- or Ab-producing cells
3.6 ELISA assay for detection of antigen-specific antibodies
3.7 In vivo assessment of T cell responses
3.8 Immunohistochemical detection of cytokines in tissues
4.0 MOLECULAR ANALYSIS OF CYTOKINE mRNA EXPRESSION
4.1 Northern blotting
4.1.1 Purification of cellular RNA
4.1.2 Electrophoresis of RNA & transfer to membranes
4.1.3 32P-labelled cDNA probe synthesis
4.1.4 Pre-hybridization, hybridization, and washing
4.1.5 Detection of mRNA bands
4.2 In Situ hybridization
4.2.1 Probe synthesis & purification
4.2.2 Preparation of slides for hybridization
4.2.3 Hybridization of 35S-cRNA riboprobes to cellular mRNA
4.2.4 Post-hybridization washing & autoradiography
4.2.5 Autoradiograph development & counter-staining
4.3 Semi-quantitative RT-PCR to detect cytokine mRNA
4.3.1 First strand cDNA Synthesis using Oligo(dT) priming
4.3.2 PCR amplification of the target cDNA
4.3.2 Detection of RT-PCR products


APPENDICES

5.1 APPENDIX A -- GENERAL METHODS
5.2 APPENDIX B -- REAGENTS & SOLUTIONS
5.3 APPENDIX C -- TISSUE CULTURE MEDIA
5.4 APPENDIX D -- MAINTENANCE OF CELL LINES

5.6 APPENDIX F -- Human cytokine RT-PCR primers
5.7 APPENDIX G -- WWW Immunology sites of interest
5.7 APPENDIX G -- Selected templates for 96--well plates

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