Vaccinia Virus and Poxvirology: Methods and Protocols

Vaccinia Virus and Poxvirology: Methods and Protocols
by Stuart N. Isaacs (Editor)

Product Details:
* Hardcover: 416 pages
* Publisher: Humana Press; 1 edition (April 19, 2004)
* Language: English
* ISBN-10: 1588292290

Book Description
A comprehensive collection of cutting-edge methods to study and work with the vaccinia virus and other poxvirus gene transcriptions. These readily reproducible techniques can be used for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; for the investigation of the binding, entry, and movement of the virus in host cells; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxviruses-the latter a critical ability given the important role of vaccinia virus in smallpox vaccination and their potential role as vaccine vectors directed against infectious agents and cancer.

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Sterile Filtration: A Practical Approach

Sterile Filtration: A Practical Approach
by Maik W. Jornitz (Author), Theodore H. Meltzer (Author)

Product Details:
* Hardcover: 623 pages
* Publisher: Informa Healthcare; 1st edition (January 15, 2001)
* Language: English
* ISBN-10: 0824702824

Book Description
This book focuses on sterilizing grade filters in the biopharmaceutical industry, emphasizing practical applications of universal and dependable operational protocols, integrity testing, and troubleshooting to streamline the production and preparation of pharmaceuticals. Addresses the complexities of globalizing redundancy in filtration! Enabling educated developmental, procedural, and regulatory judgments to be made in the manufacturing of sterile health care products, Sterile Filtration · considers how many filters should be in the process stream · examines the existence of nanobacteria and viable but nonculturable organisms · covers pore size designations, distributions, architecture, and numbers · discusses the latest findings in bubble point and diffuse flow measurements · describes pre- and postfiltration, up- and downstream testing, and after-stream sterilizations · details wetting liquid, polymer, temperature, and water purity effects · explains sieve retention, size exclusion, adsorptive sequestrations, charge-related phenomena, gravitational settling, and interference factors in liquids and gases · outlines filter validation, requirements, and operational specifics · and more! Advocating separation in addition to physical destruction of microorganisms, Sterile Filtration is a reference essential for pharmaceutical scientists; biotechnologists; microbiologists; virologists; process and chemical engineers; plant, production, validation, and quality control managers in the pharmaceutical and biotechnology industries; and upper-level undergraduate and graduate school students in these disciplines.

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