Cellular Electron Microscopy

Cellular Electron Microscopy, Volume 79 (Methods in Cell Biology)
by J. Richard McIntosh (Editor)

Product Details:
* Hardcover: 880 pages
* Publisher: Academic Press; 1 edition (February 22, 2007)
* Language: English
* ISBN-10: 0123706475

Book Description:
Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The books five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems.
* Updates the numerous technological innovations that have improved the capabilities of electron microscopy
* Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging
* Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going

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PCR/RT- PCR in situ: Light and Electron Microscopy

PCR/RT- PCR in situ: Light and Electron Microscopy (Methods in Visualization)
by Gerard Morel (Author), Mireille Raccurt (Author)

Product Details:
* Hardcover: 432 pages
* Publisher: CRC (September 27, 2002)
* Language: English
* ISBN-10: 084930041X

Book Description
This book covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and mRNA in tissues and cell cultures at the light and electron microscope levels. Written by experts in this field, the text provides theoretical consideration, as well as practical approaches to in situ PCR. Organized in a user-friendly two-column format, it provides step-by-step protocols, preparation of reagents and solutions, and appropriate controls to allow the researcher to perform and optimize these sensitive and powerful techniques.

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