Food Microbiology Protocols

Food Microbiology Protocols (Methods in Biotechnology)
by John F. T. Spencer (Editor), Alicia L. Ragout de Spencer (Editor)

Product Details:
* Hardcover: 512 pages
* Publisher: Humana Press; 1st edition (December 12, 2000)
* Language: English
* ISBN-10: 089603867X

Book Description
Expert laboratorians present a wide ranging set of detailed techniques for investigating the nature, products, and extent of the microorganisms involved in the manufacture and spoilage of foodstuff. The methods cover pathogenic organisms that cause spoilage, microorganisms in fermented foods, and microorganisms producing metabolites that affect the flavor or nutritive value of foods. Included in the section dealing with fermented foods are procedures for the maintenance of lactic acid bacteria, the isolation of plasmid and genomic DNA from species Lactobacillus, and the determination of proteolytic activity of lactic acid bacteria. A substantial number of chapters are devoted to yeasts, their use in food and beverage production, and techniques for improving industrially important strains. Comprehensive and timely, Food Microbiology Protocols is a gold-standard collection of readily reproducible techniques essential for the study of the wide variety of microorganisms involved in food production, quality, storage, and preservation today.

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RNA Modification

RNA Modification, Volume 425 (Methods in Enzymology)
by Jonatha Gott (Editor)

Product Details:
* Hardcover: 480 pages
* Publisher: Academic Press; 1 edition (August 6, 2007)
* Language: English
* ISBN-10: 0123741556

Book Description
The presence of modified nucleotides in cellular RNAs has been known for decades and over 100 distinct RNA modifications have been characterized to date. While the exact role of many of these modifications is still unclear, many are highly conserved across evolution and most contribute to the overall fitness of the organism. In recent years, new methods and bioinformatics approaches have been developed for the dissection of modification pathways and functions. These methods intersect a number of related fields, ranging from RNA processing to comparative genomics and systems biology. In addition, many of the techniques described in this volume have broad applicability, particularly in regards to the isolation, characterization, and reconstitution of ribonucleoprotein complexes, expanding the experimental repertoire available to all RNA researchers.

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