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PCR Cloning Protocols

PCR Cloning Protocols (Methods in Molecular Biology)
by Harry W. Janes, Bing-Yuan Chen (Editor)

Product Details
* Hardcover: 421 pages
* Publisher: Humana Press; 2nd edition (April 2002)
* Language: English
* ISBN-10: 0896039692
* ISBN-13: 978-0896039698

Book Description
This completely revised second edition provides proven protocols for PCR cloning and mutagenesis. It builds on its best-selling predecessor to allow researchers of all levels to increase their success rate in their experiments. The editor has divided the book into five parts, beginning with performing and optimizing PCR to Cloning PCR products, through to mutagenesis and recombination. The final two parts cover the cloning of unknown neighboring DNA and DNA library construction and screening. Each part includes an overview that summarizes the current methods available and their underlying strategies, advantages, and disadvantages for the particular research. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs. --This text refers to the Plastic Comb edition.

Book Info
Rutgers Univ., New Brunswick, NJ. Covers performing and optimizing PCR, cloning PCR products, mutagenesis and recombination, cloning unknown neighboring DNA, and library construction and screening. Features reproducible methods for isolating genes from all biological samples. Hardcover, comb-bound not yet available.

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PCR Protocols

PCR Protocols (Methods in Molecular Biology)
by John M. S. Bartlett (Editor), David Stirling (Editor)

Product Details
* Plastic Comb: 556 pages
* Publisher: Humana Press; 2nd edition (August 2003)
* Language: English
* ISBN-10: 0896036278
* ISBN-13: 978-0896036277

Book Description
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Book Info
Glasgow Royal Infirmary, UK. Features reproducible PCR techniques, genome profiling by SAGE, in situ PCR methods, tips on troubleshooting, and advice on the interpretation and use of results. Plastic-comb binding, hardcover also available.

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Cytokine Protocols

Cytokine Protocols [Methods in Molecular Biology]

Product Details:
Publisher: Humana Press
Pub Date: October 2003
Pages: 248
ISBN: 0-89603-948-X
Size: 1.76mb

Abstract:

A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization.

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Manual of Diagnostic Ultrasound in Infectious Tropical Diseases

Manual of Diagnostic Ultrasound in Infectious Tropical Diseases
by Harald T. Lutz (Editor), Hassen A. Gharbi (Editor)

Product Details
* Paperback: 180 pages
* Publisher: Springer; 1 edition (October 26, 2005)
* Language: English
* ISBN-10: 3540244468
* ISBN-13: 978-3540244462

Book Description
This manual is intended to fill a gap in the range of books on ultrasound diagnosis, concentrating exclusively on the diagnosis of infectious and tropical diseases. The book starts with a short introduction to ultrasound diagnosis. The main part describes and discusses the organ-related changes that can be detected with ultrasound in infectious and inflammatory diseases. The infectious and parasitic diseases that can successfully be diagnosed with ultrasound are presented in more detail. The important potential of interventional ultrasound diagnosis and therapy in infectious diseases is described. This richly illustrated volume has been written by leading experts from all over the world. It is primarily aimed at users of ultrasound diagnostic instruments in tropical and subtropical countries but will be valuable for physicians anywhere in the world confronted with these diseases.

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Samter's Immunologic Diseases

Samter's Immunologic Diseases
by K. F Austen MD,Michael M Frank MD,John P Atkinson MD,Harvey I Cantor MD

Product Details:
Edition: Sixth
ISBN-10: 0-7817-2120-2
ISBN-13: 978-0-7817-2120-2
Pub Date: September 2001
Pages: 1344
Illustrations: 511

The thoroughly updated Sixth Edition of this classic two-volume work continues to provide a comprehensive overview of immunology, its clinical applications, immunopathology, and allergy. A text revised and updated throughout facilitates an understanding of the host mechanisms in immunologic diseases. The strength of this text is its clinically oriented emphasis on immunologic diseases and differential approach to symptoms and syndromes. This edition features an increased clinical coverage and is an essential reference for all clinical immunologists, rheumatologists, and allergists. It will include a CD-ROM bound in the back of the book.

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Manual of Allergy and Immunology Diagnosis and Therapy

Manual of Allergy and Immunology Diagnosis and Therapy
Author(s):Daniel C Adelman MD,Jonathan Corren MD,Thomas B Casale MD

Product Details:
Edition: Fourth
Price: $49.95
ISBN-10: 0-7817-3052-X
ISBN-13: 978-0-7817-3052-5
Pub Date: February 2002
Pages: 528
Illustrations: 95

Completely revised and updated for its Fourth Edition, this Spiral(R) Manual is a quick-reference guide to the diagnosis and treatment of allergies and immunologic disorders in children and adults. Coverage includes allergic and immunologic disorders of each organ system; anaphylaxis; insect, drug, and food allergies; rheumatic diseases; immunohematology; transplantation immunology; primary immunodeficiency diseases; HIV disease and related opportunistic infections; immunologic diagnostic techniques; and immunization and immunoprophylaxis.

The book is written in an easy-to-scan outline format, with numerous tables. Fourteen appendices provide rapid access to essential information, including normal laboratory values, allergy elimination diets, and manufacturers of allergenic extracts and environmental control products.

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Principles and Practice of Infectious Diseases (CHM Version)

Principles and Practice of Infectious Diseases 6th Edition

Product Details
# Hardcover: 4000 pages
# Publisher: Churchill Livingstone; 6 edition (October 22, 2004)
# Language: English
# ISBN-10: 0443066434
# ISBN-13: 978-0443066436
# Format: CHM
# Size: 54 Mb
Widely acknowledged as the definitive reference source of its kind, Principles and Practice of Infectious Diseases is back - in a new 6th Edition! Authored by a "who's who" of experts from around the world, this 2-volume set encompasses all that is currently known about the epidemiology, aetiology, pathology, microbiology, immunology, and treatment of infectious agents. Completely revised and updated - and with a bold, new 4-colour format - it is a complete, essential compendium of knowledge on this vast and complex subject.

This Book has been already been posted in PDB version

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