PCR Protocols

PCR Protocols (Methods in Molecular Biology)
by John M. S. Bartlett (Editor), David Stirling (Editor)

Product Details
* Plastic Comb: 556 pages
* Publisher: Humana Press; 2nd edition (August 2003)
* Language: English
* ISBN-10: 0896036278
* ISBN-13: 978-0896036277

Book Description
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Book Info
Glasgow Royal Infirmary, UK. Features reproducible PCR techniques, genome profiling by SAGE, in situ PCR methods, tips on troubleshooting, and advice on the interpretation and use of results. Plastic-comb binding, hardcover also available.

Download