Inflammation Protocols

Inflammation Protocols (Methods in Molecular Biology)
by Paul G. Winyard (Editor), D. A. Willoughby (Editor)

Product Details
* Hardcover: 378 pages
* Publisher: Humana Press (April 2003)
* Language: English
* ISBN-10: 0896039706
* ISBN-13: 978-0896039704

Book Info
Univ. of Exeter and Plymouth, UK. Text covers techniques for evaluating inflammatory disease processes and for discovering new drugs for treating chronic human inflammatory diseases. For experimental and clinical researchers. Illustrated.

Table of Contents

Part I. In Vitro Systems for Studying Aspects of the Inflammatory Response and Testing Antiinflammatory Drugs

Key Stages in the Acute Inflammatory Response and Their Relevance as Therapeutic Targets: Introduction to Part 1
Paul G. Winyard

IkB Kinase and NF-kB Signaling in Response to Pro-Inflammatory Cytokines
Mireille Delhase

Screening for Inhibitors of Transcription Factors, Using Luciferase Reporter Gene Expression in Transfected Cells
Deborah Phippard and Anthony M. Manning

Adhesion Molecule Expression on Cytokine-Stimulated Human Endothelial Cells
Susan L. Cuvelier and Kamala D. Patel

Phagocytosis by Inflammatory Phagocytes: Experimental Strategies for Stimulation and Quantification
M. Rachel Morris, Sharon Dewitt, Iraj Laffafian, and Maurice B. Hallett

Cytosolic Ca2+ Measurement and Imaging in Inflammatory Cells
Sharon Dewitt, Iraj Laffafian, M. Rachel Morris, and Maurice B. Hallett

Detection and Visualization of Oxidase Activity in Phagocytes
Maurice B. Hallett, Caroline Cole, and Sharon Dewitt

Measurement of Complement Activation
Tom Eirik Mollnes

Measurement of Matrix Metalloproteinase Activities in the Medium of Cultured Synoviocytes Using Zymography
Linda Troeberg and Hideaki Nagase

Measurement of Aggrecanase-Generated Interglobular Domain Catabolites in the Medium and Extracts of Cartilage Explants Using Western Blot Analysis
Clare E. Hughes, Christopher B. Little, and Bruce Caterson

In Vitro Model of Human Articular Cartilage Degradation
William D. Shingleton

Part II. In Vivo Models of Inflammation

In Vivo Models of Inflammation: Introduction to Part 2
Derek A. Willoughby

Carrageenan-Induced Paw Edema in the Rat and Mouse
Christopher J. Morris

Pleural Models of Inflammation: Immune and Nonimmune
Adrian R. Moore

Models of Acute Inflammation in the Ear
Miklós Gábor

Migration of Specific Leukocyte Subsets in Response to Cytokine or Chemokine Application In Vivo
Mauro Perretti and Stephen J. Getting

Inflammatory Joint Disease: Clinical, Histological, and Molecular Parameters of Acute and Chronic Inflammation and Tissue Destruction
Nancy L. McCartney-Francis, James Chan, and Sharon M. Wahl

The Assessment of Inflammation, Cartilage Matrix, and Bone Loss in Experimental Monoarticular Arthritis of the Rat
Michael P. Seed

Collagen-Induced Arthritis
Adrian R. Moore

Air-Pouch Models of Inflammation and Modifications for the Study of Granuloma-Mediated Cartilage Degradation
Paul Colville-Nash and Toby Lawrence

Quantitative Analysis of Angiogenesis Using the Murine Chronic Granulomatous Air Pouch
Chandan A. S. Alam

Models of Coronary Artery Occlusion and Reperfusion for the Discovery of Novel Antiischemic and Antiinflammatory Drugs for the Heart
Nicole S. Wayman, Michelle C. McDonald, Prabal K. Chatterjee, and Christoph Thiemermann

Assessment of Anticolitic Drugs in the Trinitrobenzene Sulfonic Acid (TNBS) Rat Model of Inflammatory Bowel Disease
Brendan J. R. Whittle, Maryan Cavicchi, and Dominique Lamarque

An In Vivo Model of Ischemia/Reperfusion and Inflammation of the Kidneys of the Rat
Prabal K. Chatterjee and Christoph Thiemermann

In Vivo Models of Inflammation: Immune Rejection and Skin Transplantation In Vivo
Isabelle Binet and Kathryn J. Wood

Wound Healing: A Model of Dermal Wound Repair
Annette Tomlinson and Mark W. J. Ferguson

Part III. Pharmacodynamic Endpoints in Experimental Models and in Clinical Studies in Humans

An Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans: Introduction to Part 3
David R. Blake and Gordon J. Taylor

A Reply to “An Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans”
Derek A. Willoughby

Quantifying Inflammation In Vivo Using Radiolabeled Antibodies and Leukocytes
Diane Marshall and Dorian O. Haskard

Immunoperoxidase Histochemistry for the Detection of Cellular Adhesion Molecule, Cytokine, and Chemokine Expression in the Arthritic Synovium
Zoltan Szekanecz and Alisa E. Koch

Roles of Nitric Oxide and Superoxide in Inflammation
Daniela Salvemini, Harry Ischiropoulos, and Salvatore Cuzzocrea

Analysis of Nitrite and Nitrate in the Study of Inflammation
Claire A. Davies, Sophie A. Rocks, Meg C. O'Shaughnessy, David Perrett, and Paul G. Winyard

In Vivo Assays for COX-2
Chi-Chung Chan

Measurement of 8-epi-PGF2a as a Marker of Lipid Peroxidation In Vivo by Immunoaffinity Extraction and Gas Chromatography-Mass Spectrometry
Nitin K. Gopaul and Erik E. Änggård

Laboratory Assessment of the Acute Phase Response: Using CRP as a Model, Robert F. Ritchie and Thomas B. Ledue

Assays of Matrix Metalloproteinases (MMPs) and MMP Inhibitors: Bioassays and Immunoassays Applicable to Cell Culture Medium, Serum, and Synovial Fluid
Jon B. Catterall and Tim E. Cawston

Index

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Handbook of Media for Clinical Microbiology

Handbook of Media for Clinical Microbiology, Second Edition
by Atlas M. (Author)

Product details
* Hardcover: 544 pages
* Publisher: CRC Press Inc (17 May 2006)
* Language English
* ISBN-10: 084933795X
* ISBN-13: 978-0849337956

Book Info:
Synopsis
While evolving molecular diagnostic methods are being heralded for the role they will play in improving our ability to cultivate and identify bacteria, fungi, and viruses, the reality is that those new methods are still beyond the technical and financial reach of most clinical laboratories. Most clinical microbiology laboratories still rely upon culture methods for the identification of microorganisms of medical importance. The newest edition of the "Handbook of Media for Clinical Microbiology" addresses the needs of clinical microbiology laboratories and infectious disease researchers. Authored by Ronald Atlas and James Snyder, who over the years have built solid reputations among researchers for their exceptionally reliable media handbooks, this volume gives microbiologists in clinical and medical laboratories the reference they need to quickly and effectively deal with the modern challenges shaping the field. Organized for a fast-paced environment, this fully updated Second Edition provides clinical diagnostic laboratories with an easy-to-use reference for those routine and specialized media employed in the cultivation of pathogenic bacteria, fungi, and viruses. Almost 1,650 media are described in this edition, among them many newly developed media designed for the rapid detection and identification of disease-causing microorganisms, including those responsible for emerging and re-emerging infectious diseases. It describes media designed to cultivate and identify Escherichia coli O157:H7, methicillin-resistant Staphylococcus aureus, and vancomycin-resistant enterococci. It also includes chromogenic or fluorogenic substrates that permit the rapid detection of specific pathogens critical to the diagnosis of individuals with specific infectious diseases. The Handbook provides a compilation of the formulations, methods of preparation, and applications for media used in the clinical microbiology laboratory. Listings are alphabetical, and each includes medium composition, instruction for preparation, commercial sources, and intended uses. This highly useful resource also discusses the role of the clinical laboratory in the diagnosis of infectious diseases and the process for detecting infectious diseases, including specimen receipt, media selection, antibiotic susceptibility testing, and biosafety considerations. It includes media for the cultivation of emerging and re-emerging pathogens and bioterrorism threat agents.

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