Electron Microscopy: Methods and Protocols

Electron Microscopy: Methods and Protocols (Methods in Molecular Biology)
by John Kuo (Editor)

Product Details
* Hardcover: 670 pages
* Publisher: Humana Press; 2nd ed. edition (February 27, 2007)
* Language: English
* ISBN-10: 1588295737

Book Description
In this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Each protocol is presented by experts with years of first-hand experience and in-depth knowledge of the techniques they describe. The Notes section accompanying each protocol will prove invaluable for adapting and troubleshooting protocols.
Electron Microscopy: Methods and Protocols, Second Edition, comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.

Download

Adenovirus Methods And Protocols: Adenoviruses, Ad Vectors, Quantitation, And Animal Models

Adenovirus Methods And Protocols: Adenoviruses, Ad Vectors, Quantitation, And Animal Models (Methods in Molecular Medicine)
by William S. M. Wold (Editor), Ann E. Tollefson (Editor)

Product Details
* Hardcover: 241 pages
* Publisher: Humana Press; 2 edition (February 3, 2007)
* Language: English
* ISBN-10: 1588295982

Book Description
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.
Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.

Download