Baculovirus and Insect Cell Expression Protocols

Baculovirus and Insect Cell Expression Protocols (Methods in Molecular Biology)
by David W. Murhammer (Editor)

Product Details
Hardcover: 484 pages
Publisher: Humana Press; 2nd ed. edition (July 6, 2007)
Language: English
ISBN-10: 1588295370

Book Description
Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

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