Diagnostic Bacteriology Protocols

Diagnostic Bacteriology Protocols :Methods in Molecular Biology, vol.46
Editor - Howard, Jenny; Whitcombe, David M.
Humana Press; 1995;
pp293;
ISBN: 0896032973


Diagnostic Bacteriology Protocols presents a broad range of currently used techniques for detecting, identifying, and differentiating bacterial cell components, including structured proteins, nucleic acids, enzyme activities, lipopolysaccharides, and metabolites. It describes each technique in simple easy-to-follow steps that guarantee reproducible results for novices and senior researchers alike. Troubleshooting tips, alternative ways of performing procedures, and informative explanations about why certain steps are necessary-aids not usually found in standard journal recipes help even highly skilled researchers to obtain the results they want.

Table of Contents

Antibiotic Resistance Profiling (pp. 1-4)Hammonds, Stephen J.

Bacteriocin Typing (pp. 5-14)Pitt, Tyrone L.; Gaston, Michael A.

Bacteriophage Typing (pp. 15-26)Pitt, Tyone L.; Gaston, Michael A.
The Analysis of Bacterial Proteins by SDS Polyacrylamide Gel Electrophoresis (pp. 27-40)Costas, Menelaos

Lipopolysaccharide Chemotyping (pp. 41-48)Chart, Henrik

Analysis of Bacterial Outer Membrane Proteins (pp. 49-62)Chart, Henrik

Multilocus Enzyme Electrophoresis (pp. 63-78)Boerlin, Patrick; Piffaretti, Jean-Claude

Fast Atom Bombardment-Mass Spectrometry (pp. 79-96)Drucker, David B.

Pyrolysis Mass Spectrometry (pp. 97-106)Freeman, Roger; Sisson, Penelope R.; Heatherington, Clive S.

The Direct Immunofluorescent Filter Technique (DIFT) (pp. 107-112)Kroll, Rohan

The Direct Epifluorescent Filter Technique (DEFT) (pp. 113-122)Kroll, Rohan

Use of Commercially Available ELISA Kits for Detection of Foodborne Pathogens (pp. 123-132)Robison, Barbara J.

Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format (pp. 133-140)Groody, E. Patrick

Preparation of Bacterial Genomic DNA (pp. 141-148)Bickley, Jane; Owen, Robert J.

Pulsed Field Gel Electrophoresis (pp. 149-164)Hillier, Alan J.; Davidson, Barrie E.

Application of Total DNA Restriction Pattern Analysis to Identification and Differentiation of Bacterial Strains (pp. 165-180)Giovannetti, Luciana; Ventura, Stefano

Determination of rRNA Gene Restriction Patterns (pp. 181-200)Grimont, Francine; Grimont, Patrick A. D.

Preparation of Nonradioactive DNA Probes (pp. 201-212)Eley, Adrian; Oxley, Kevin M.

Preparation of Bacterial Plasmid DNA (pp. 213-224)Tully, Michael

Plasmid Profile Typing and Plasmid Fingerprinting (pp. 225-236)Threlfall, E. John; Woodford, Neil

Polymerase Chain Reaction for the Detection of Listeria Species and Listeria monocytogenes (pp. 237-246)Siggens, Kenneth W.

Development of Bacterial Species-Specific DNA Probes Based on Ribosomal RNA Genes Using PCR (pp. 247-256)Smith, Terry J.; Maher, Majella; Gannon, Frank; Dawson, Michael T.

Identification of Microorganisms Using Random Primed PCR (pp. 257-268)Mileham, Alan J.
Ligase Chain Reaction (pp. 269-278)Shimer, George H.; Backman, Keith C.

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