E.Coli Gene Expression Protocols

E. coli Gene Expression Protocols (Methods in Molecular Biology)
by Peter E. Vaillancourt

Product Details
Hardcover: 347 pages
Publisher: Humana Press; 1st edition (October 15, 2002)
Language: English
ISBN: 1588290085

Book Info
Applied Molecular Evolution, San Diego, CA. Provides a state-of-the-art collection of tested methods for this gene expression technology and offers researchers tools for success in the emerging fields of functional genomics and proteomics.

First Sentence:
Rapid transfer of exponentially growing E. coli cultures from physiological to low temperatures (10-15 C) has profound consequences on cell physiology: membrane fluidity decreases, which interferes with transport and secretion, the secondary structures of nucleic acids are stabilized, which affect the efficiencies of mRNA transcription/translation and DNA replication, and free ribosomal sub-units and 70S particles accumulate at the expense of polysomes, negatively impacting translation of most cellular mRNAs.

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