In Situ Hybridization Protocols

In Situ Hybridization Protocols (Methods in Molecular Biology)
by K. H. Andy Choo (Editor)

Product Details
* Paperback: 497 pages
* Publisher: Humana Press; Spiral edition (September 1994)
* Language: English
* ISBN-10: 0896032809
* ISBN-13: 978-0896032804

Table of Contents

Preparation of Human Chromosomal Painting Probes from Somatic Cell Hybrids. Reverse Chromosome Painting. FISH Detection on DAPI Banded Chromosomes. Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerase Chain Reaction. In Situ Hybridization Using Synthetic Oligomers as Probes for Centromere and Telomere Repeats. Mapping Human YAC Clones by Fluorescence In Situ Hybridization using Alu-PCR from Single Yeast Colonies. FISH of Alu-PCR Amplified YAC Clones and Applications in Tumor Cytogenetics. Primed In Situ (PRINS) Labeling of DNA. Free Chromatin Mapping by FISH. Use of DNA-HALO Preparations for High-Resolution DNA In Situ Hybridization. In Situ Hybridization of Meiotic Prophase Chromosomes. Strand-Specific Fluorescence In Situ Hybridization for Determining Orientation and Direction of DNA Sequences. Radioactive In Situ Hybridization to Replication-banded Chromosomes. Gene Mapping Using 3H-labeled Heterologous Probes. Dual-Label Fluorescence In Situ Hybridization Detection of Individual Chromosomes in Sperm Using Ejaculated Semen. In Situ hybridization to Drosophila Polytene Chromosomes. In Situ Hybridization to Polytene Chromosomes of Drosophila Melanogaster and Other Dipteran Species. Microsurgical Isolation of Native Polytene Chromosomes of Drosophila Melanogaster for the In Situ Observation of Conformation and Distribution of Molecules. PCR In Situ Hybridization. Detection of Virus Nucleic Acids by Radioactive and Nonisotopic In Situ Hybridization. Detection of Viral Genomes. Primed In Situ (PRINS) Labeling of RNA. Hybridization Histochemistry Using Radiolabeled Oligodeoxyribonucleotide Probes. mRNA In Situ Hybridization to In Vitro Cultured Cells. Quantitative In Situ Hybridization Using Radioactive Probes in the Study of Gene Expression in Heterocellular Systems. Tissue Distribution of Gene Expression in Mammalian Development. Subcellular Location of mRNA by Electron Microscope Hybridization Histochemistry. Principle of Digital Imaging Microscopy. Application of In Situ Hybridization for the Detection of Virus Nucleic Acids. Positional Cloning and Multicolor In Situ Hybridization: Principles and Protocols. Characterization of Constitutive Marker Chromosomes in Humans. Index.

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