Lymphocytes: A Practical Approach

Lymphocytes: A Practical Approach (Practical Approach Series)
by Sarah L. Rowland-Jones (Editor), Andrew J. McMichael (Editor)

Product Details:
* Hardcover: 352 pages
* Publisher: Oxford University Press, USA; 2 edition (July 24, 2002)
* Language: English
* ISBN-10: 0199638179


Book Description
Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discouraged by the complicated protocols involved in cellular immunological studies, as illustrated by the meticulous care required for the generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.

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